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  Evolution and single-droplet analysis of fuel-driven compartments by droplet-based microfluidics

Bergmann, A. M., Donau, C., Späth, F., Jahnke, K., Göpfrich, K., & Boekhoven, J. (2022). Evolution and single-droplet analysis of fuel-driven compartments by droplet-based microfluidics. Angewandte Chemie International Edition, 61(32): e202203928, pp. 1-8. doi:10.1002/anie.202203928.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000B-322B-D Version Permalink: https://hdl.handle.net/21.11116/0000-000B-3232-4
Genre: Journal Article

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 Creators:
Bergmann, Alexander M., Author
Donau, Carsten, Author
Späth, Fabian, Author
Jahnke, Kevin1, Author           
Göpfrich, Kerstin1, Author           
Boekhoven, Job, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: Artificial Organelles; Droplet-Based Microfluidics; Nonequilibrium Processes; Phase Transitions
 Abstract: Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short timescales of their kinetics. We used droplet-based microfluidics to encapsulate a fuel-driven cycle that drives phase separation into coacervate-based droplets to overcome this challenge. This approach enables the analysis of every coacervate-based droplet in the reaction container throughout its lifetime. We discovered that the fuel concentration dictates the formation of the coacervate-based droplets and their properties. We observed that coacervate-based droplets grow through fusion, decay simultaneously independent of their volume, and shrinkage rate scales with their initial volume. This method helps to further understand the regulation of membraneless organelles, and we believe the analysis of individual coacervate-based droplets enables future selection- or evolution-based studies.

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Language(s): eng - English
 Dates: 2022-03-162022-06-032022-06-242022-08-08
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://pubmed.ncbi.nlm.nih.gov/35657164/
DOI: 10.1002/anie.202203928
 Degree: -

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 61 (32) Sequence Number: e202203928 Start / End Page: 1 - 8 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851