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  Decision making and fMRI responses modulated by remote magnetogenetics stimulation in rats

Israeli, R., Johnson, A., Chen, Y., Qian, C., Pakray, S., Gilad, A., et al. (2022). Decision making and fMRI responses modulated by remote magnetogenetics stimulation in rats. In 51st Annual Meeting of the Society for Neuroscience (Neuroscience 2022).

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Genre: Meeting Abstract

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 Urheber:
Israeli, RR, Autor
Johnson, AW, Autor
Chen, Y1, Autor           
Qian, C, Autor
Pakray, SA, Autor
Gilad, AA, Autor
Pelled, G, Autor
Affiliations:
1Research Group Translational Neuroimaging and Neural Control, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_2528695              

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 Zusammenfassung: Neuromodulation is critical in studying and treating many neuronal diseases and disorders; however, it relies on techniques that are often either invasive or nonspecific. We are developing a technology to noninvasively activate specific neurons using electromagnetic fields and an electromagnetic perceptive gene (EPG). Glass catfish (Kyptopterus vitreolus) has a gene that encodes a protein responsive to magnetic fields. This gene (EPG) has been isolated, cloned, and expressed in mammalian cells. EPG can be used through viral injection to target specific neurons which can then be noninvasively stimulated using electromagnetic fields (Krishnan et al., 2018). Our research focuses on the modulating neurons in the visual cortex since it is widely studied and can be researched by behavioral experiments and through functional imaging. Long Evans rats were genetically engineered to express EPG in excitatory neurons of the primary visual cortex via stereotaxic injection of adeno-associated virus containing EPG under CaMKII promoter. These rats were then tested for behavioral responses to magnetic stimulus using an operant conditioning chamber. While in the chamber, an electromagnetic coil attached to the rat’s head delivered 50 mT of magnetic field for 2 seconds every minute, repeated for 25 trials. The sham stimulus included the same electromagnetic coil attached to the rat’s head with the same electric current flowing, but in opposing direction, eliminating the magnetic field. In response to magnetic stimulus, rats pressed a lever and received a sucrose solution reward. Once the rats learned to associate the stimulus with pressing the lever, the same experiment was conducted using the sham stimulus. Preliminary data has shown significantly (p<0.05) faster responses when receiving an electromagnetic stimulation as compared to sham stimulation. Additionally, we used a 7T Bruker MRI to perform fMRI on rats expressing EPG in their visual cortex. Using fiber optic cables, we stimulated both eyes with a 5Hz flashing light for 20 seconds. While both control and EPG rats showed significant activation in the superior colliculus, only EPG rats had significant activation in the both hemispheres of the visual cortex (p<0.01), corresponding to the region of neurons containing the EPG. This may suggest that EPG increases activity and connectivity between neurons when under the strong magnetic fields of the MRI. Overall, these results suggest that EPG may be a new method to noninvasively activate specific neurons.

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 Datum: 2022-11
 Publikationsstatus: Online veröffentlicht
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Titel: 51st Annual Meeting of the Society for Neuroscience (Neuroscience 2022)
Veranstaltungsort: San Diego, CA, USA
Start-/Enddatum: 2022-11-12 - 2022-11-16

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Titel: 51st Annual Meeting of the Society for Neuroscience (Neuroscience 2022)
Genre der Quelle: Konferenzband
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Seiten: - Band / Heft: - Artikelnummer: 183.04 Start- / Endseite: - Identifikator: -