Genome mining enabled by biosynthetic characterization uncovers a class of 
benzoxazolinate-containing natural products in diverse bacteria

Shi, Y.-M.; Crames, J. J.; Czech, L.; Bozhüyük, K. A. J.; Shi, Y.-N.; Hirschmann, M.; Lamberth, S.; Claus, P.; Paczia, N.; Ruckert, C.; Kalinowski, J.; Bange, G.; Bode, H. B.

doi:10.1002/anie.202206106

Genome mining enabled by biosynthetic characterization uncovers a class of benzoxazolinate-containing natural products in diverse bacteria

Shi, Y.-M., Crames, J. J., Czech, L., Bozhüyük, K. A. J., Shi, Y.-N., Hirschmann, M., Lamberth, S., Claus, P., Paczia, N., Ruckert, C., Kalinowski, J., Bange, G., & Bode, H. B. (2022). Genome mining enabled by biosynthetic characterization uncovers a class of benzoxazolinate-containing natural products in diverse bacteria. Angewandte Chemie, International Edition in English, 61(51):. doi:10.1002/anie.202206106.

Item is 公開

表示: 全項目非表示: 全項目

基本情報

表示: 非表示:

アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000B-3ED7-E 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0010-3987-6

資料種別: 学術論文

ファイル

表示: ファイル

作成者

表示:

非表示:

作成者:
Shi, Y.-M.¹, 著者
Crames, J. J.², 著者
Czech, L., 著者
Bozhüyük, K. A. J.², 著者
Shi, Y.-N.², 著者
Hirschmann, M., 著者
Lamberth, S., 著者
Claus, P.³, 著者
Paczia, N.³, 著者
Ruckert, C., 著者
Kalinowski, J., 著者
Bange, G.⁴, 著者
Bode, H. B.^{2, 5, 6, 7}, 著者

所属:
1Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266281
2Natural Product Function and Engineering, Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266308
3Core Facility Metabolomics and small Molecules Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266267
4Max Planck Fellow Molecular Physiology of Microbes, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3321791
5Senckenberg Gesellschaft für Naturforschung, Frankfurt, ou_persistent22
6Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Frankfurt, Germany, External Organizations, ou_421891
7Chemical Biology, Department of Chemistry, Philipps University Marburg, Marburg, Germany, ou_persistent22

内容説明

表示:

非表示:

キーワード: Natural products biosynthesis heterocycles non-proteinogenic amino acids non-ribosomal peptide synthetase

要旨: Benzoxazolinate is a rare bis-heterocyclic moiety that interacts with proteins and DNA and confers extraordinary bioactivities on natural products, such as C-1027. However, the biosynthetic gene responsible for the key cyclization step of benzoxazolinate remains unclear. Here, we show a putative acyl AMP-ligase responsible for the last cyclization step. We then use the enzyme as a probe for genome mining and discover that the orphan benzobactin gene cluster in entomopathogenic bacteria prevails across Proteobacteria and Firmicutes. It turns out that Pseudomonas chlororaphis produces various benzobactins, whose biosyntheses arehighlighted by a synergistic effect of two unclustered genes encoding enzymes on boosting benzobactin production; the formation of non-proteinogenic 2-hydroxymethylserine by a serine hydroxymethyltransferase; and the types I and II NRPS architecture for structural diversity. Our findings reveal the biosynthetic potential of a widespread benzobactin gene cluster.

資料詳細

表示:

非表示:

言語: eng - English

日付: 出版: 2022-10-06

出版の状態: 出版

ページ: -

出版情報: -

目次: -

査読: 査読あり

識別子（DOI, ISBNなど）: その他: 36198080
DOI: 10.1002/anie.202206106
ISSN: 1521-3773 (Electronic)1433-7851 (Linking)

学位: -

訴訟

表示:

Project information

表示: 非表示:

Project name : LOEWE Centre for Translational Biodiversity Genomics (LOEWE TBG)

Grant ID : -

Funding program : Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz (LOEWE)

Funding organization : State of Hesse

Project name : ERC advanced grant SYNPEP

Grant ID : 835108

Funding program : Horizon 2020 (H2020)

Funding organization : European Research Council (EC)

Project name : Open Access

Grant ID : -

Funding program : -

Funding organization : Project DEAL

出版物 1

表示:

非表示:

出版物名: Angewandte Chemie, International Edition in English

省略形 : Angew. Chem., Int. Ed. Engl.

種別: 学術雑誌

著者・編者:

所属:

出版社, 出版地: Weinheim : Wiley-VCH

ページ: - 巻号: 61 (51) 通巻号: e202206106 開始・終了ページ: - 識別子（ISBN, ISSN, DOIなど）: ISSN: 0570-0833
CoNE: https://pure.mpg.de/cone/journals/resource/0570-0833

アイテム詳細

基本情報

ファイル

関連URL

作成者

内容説明

資料詳細

関連イベント

訴訟

Project information

出版物 1