Transformation of agrobacterium using the freeze-thaw method

Weigel, D; Glazebrook, J

doi:10.1101/pdb.prot4666

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Transformation of agrobacterium using the freeze-thaw method

Weigel, D., & Glazebrook, J. (2006). Transformation of agrobacterium using the freeze-thaw method. Cold Spring Harbor protocols, 2006(7): pdb.prot4666. doi:10.1101/pdb.prot4666.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000B-44F6-3 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-44F7-2

Genre: Journal Article

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Weigel, D¹, Author
Glazebrook, J, Author

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1Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375790

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Abstract: This protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for Escherichia coli, it is possible to obtain adequate numbers of transformants with this technique.

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Dates: Date issued: 2006-12

Publication Status: Issued

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Identifiers: DOI: 10.1101/pdb.prot4666
PMID: 22484682

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Title: Cold Spring Harbor protocols

Abbreviation : CSH Protocols

Source Genre: Journal

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Publ. Info: NY : Cold Spring Harbor

Pages: - Volume / Issue: 2006 (7) Sequence Number: pdb.prot4666 Start / End Page: - Identifier: ISSN: 1559-6095
CoNE: https://pure.mpg.de/cone/journals/resource/1559-6095