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  The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas

Vidal-Meireles, A., Kuntam, S., Széles, E., Tóth, D., Neupert, J., Bock, R., & Tóth, S. Z. (2023). The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas. Plant, Cell and Environment, 46(2), 422-439. doi:10.1111/pce.14481.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-000B-5F28-F 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000C-3E68-B
資料種別: 学術論文
その他のタイトル : Plant, Cell & Environment

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 作成者:
Vidal-Meireles, André1, 著者
Kuntam, Soujanya1, 著者
Széles, Eszter1, 著者
Tóth, Dávid1, 著者
Neupert, J.2, 著者           
Bock, R.2, 著者           
Tóth, Szilvia Z.1, 著者
所属:
1external, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

内容説明

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キーワード: Chlamydomonas, CO2 availability, inducible amiRNA, oxygen-evolving complex, photoinhibition, photosystem II, protein lifetime, PSBO
 要旨: SummaryPSBO is essential for the assembly of the oxygen-evolving complex in plants and green algae. Despite its importance, we lack essential information on its lifetime and how it depends on the environmental conditions. We have generated nitrate-inducible PSBO amiRNA lines in the green alga Chlamydomonas reinhardtii. Transgenic strains grew normally under non-inducing conditions, and their photosynthetic performance was comparable to the control strain. Upon induction of the PSBO amiRNA constructs, cell division halted. In acetate-containing medium, cellular PSBO protein levels decreased by 60% within 24 hours in the dark, by 75% in moderate light, and in high light, the protein completely degraded. Consequently, the photosynthetic apparatus became strongly damaged, probably due to ?donor-side-induced photoinhibition?, and cellular ultrastructure was also severely affected. However, in the absence of acetate during induction, PSBO was remarkably stable at all light intensities and less substantial changes occurred in photosynthesis. Our results demonstrate that the lifetime of PSBO strongly depends on the light intensity and carbon availability, and thus, on the metabolic status of the cells. We also confirm that PSBO is required for photosystem II stability in C. reinhardtii and demonstrate that its specific loss also entails substantial changes in cell morphology and cell cycle.This article is protected by copyright. All rights reserved.

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言語: eng - English
 日付: 2022-11-012023-02
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: -
 識別子(DOI, ISBNなど): DOI: 10.1111/pce.14481
 学位: -

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出版物 1

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出版物名: Plant, Cell and Environment
  その他 : Plant, Cell & Environment
種別: 学術雑誌
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出版社, 出版地: Oxford, England : Blackwell Science
ページ: - 巻号: 46 (2) 通巻号: - 開始・終了ページ: 422 - 439 識別子(ISBN, ISSN, DOIなど): ISSN: 0140-7791
CoNE: https://pure.mpg.de/cone/journals/resource/954925471334