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  Complementation between polymerase- and exonuclease-deficient mitochondrial DNA polymerase mutants in genomically engineered flies

Bratic, A., Kauppila, T. E. S., Macao, B., Grönke, S., Siibak, T., Stewart, J. B., et al. (2015). Complementation between polymerase- and exonuclease-deficient mitochondrial DNA polymerase mutants in genomically engineered flies. Nat Commun, 6, 8808. doi:10.1038/ncomms9808.

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http://www.ncbi.nlm.nih.gov/pubmed/26554610 (beliebiger Volltext)
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 Urheber:
Bratic, A.1, Autor           
Kauppila, T. E. S.1, Autor           
Macao , B., Autor
Grönke, S.2, Autor           
Siibak, T., Autor
Stewart, J. B.3, Autor           
Baggio, F.1, Autor           
Dols, J.2, Autor           
Partridge, L.2, Autor           
Falkenberg, M.1, Autor           
Wredenberg, A.1, Autor           
Larsson, N.G.1, Autor           
Affiliations:
1Department Larsson - Mitochondrial Biology, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_1942286              
2Department Partridge - Biological Mechanisms of Ageing, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_1942287              
3Stewart – Mitochondrial Mutations and Genome Co-evolution, Research Groups, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_1942301              

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Schlagwörter: Animals DNA, Mitochondrial/*genetics DNA-Directed DNA Polymerase/genetics/*metabolism Drosophila/*genetics Exodeoxyribonucleases/genetics/*metabolism *Genetic Engineering Mutagenesis, Site-Directed Mutation Protein Subunits
 Zusammenfassung: Replication errors are the main cause of mitochondrial DNA (mtDNA) mutations and a compelling approach to decrease mutation levels would therefore be to increase the fidelity of the catalytic subunit (POLgammaA) of the mtDNA polymerase. Here we genomically engineer the tamas locus, encoding fly POLgammaA, and introduce alleles expressing exonuclease- (exo(-)) and polymerase-deficient (pol(-)) POLgammaA versions. The exo(-) mutant leads to accumulation of point mutations and linear deletions of mtDNA, whereas pol(-) mutants cause mtDNA depletion. The mutant tamas alleles are developmentally lethal but can complement each other in trans resulting in viable flies with clonally expanded mtDNA mutations. Reconstitution of human mtDNA replication in vitro confirms that replication is a highly dynamic process where POLgammaA goes on and off the template to allow complementation during proofreading and elongation. The created fly models are valuable tools to study germ line transmission of mtDNA and the pathophysiology of POLgammaA mutation disease.

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 Datum: 2015
 Publikationsstatus: Erschienen
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 Identifikatoren: DOI: 10.1038/ncomms9808
ISSN: 2041-1723 (Electronic)2041-1723 (Linking)
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Titel: Nat Commun
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 6 Artikelnummer: - Start- / Endseite: 8808 Identifikator: -