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Free keywords:
F-Box Proteins/*metabolism
GTP Phosphohydrolases/*metabolism
Membrane Fusion/*physiology
Membrane Proteins/*metabolism
Mitochondria/*metabolism
Mitochondrial Membranes/metabolism
Mitochondrial Proteins
Proteasome Endopeptidase Complex/metabolism
Protein Binding
SKP Cullin F-Box Protein Ligases/metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins/*metabolism
Time Factors
Ubiquitin/metabolism
Abstract:
Mitochondrial morphology depends on balanced fusion and fission events. A central component of the mitochondrial fusion apparatus is the conserved GTPase Fzo1 in the outer membrane of mitochondria. Mdm30, an F-box protein required for mitochondrial fusion in vegetatively growing cells, affects the cellular Fzo1 concentration in an unknown manner. We demonstrate that mitochondrial fusion requires a tight control of Fzo1 levels, which is ensured by Fzo1 turnover. Mdm30 binds to Fzo1 and, dependent on its F-box, mediates proteolysis of Fzo1. Unexpectedly, degradation occurs along a novel proteolytic pathway not involving ubiquitylation, Skp1-Cdc53-F-box (SCF) E3 ubiquitin ligase complexes, or 26S proteasomes, indicating a novel function of an F-box protein. This contrasts to the ubiquitin- and proteasome-dependent turnover of Fzo1 in alpha-factor-arrested yeast cells. Our results therefore reveal not only a critical role of Fzo1 degradation for mitochondrial fusion in vegetatively growing cells but also the existence of two distinct proteolytic pathways for the turnover of mitochondrial outer membrane proteins.
