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Free keywords:
high-throughput in-gel digest
in-gel digest and desalting platform
microplate in-gel digestion and desalting
oasis (r) hlb mu elution plate
technology
sequence-analysis
electrophoresis
Abstract:
Separation or prefractionation of proteins by gel electrophoresis, followed by in-gel proteolytic digest and analysis by MS is a typical standard application in proteomics. For many laboratories, it is not cost-effective to use a robot with all its drawbacks. On the other hand, manual digest is time consuming and not free of error if many samples are processed at the same time. The OASIS (R) HLB mu Elution plate allows the handling of 96 samples for protein in-gel digest and peptide desalting in a semiautomatic way. Using multichannel pipettes, solutions are added quickly, and with the use of the positive pressure-96 stand all solutions are removed simultaneously. In this article, a complete and detailed protocol for the use of the OASIS (R) HLB mu Elution plate is introduced and its effectiveness is shown with 2D- and 1D-gel samples.
