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Animals
Ethyl Methanesulfonate/pharmacology
Feasibility Studies
Female
Genome, Helminth/drug effects/*physiology
Genome-Wide Association Study
Mutagenesis/drug effects/*physiology
Mutagens/pharmacology
Rats
Rats, Wistar
Sequence Analysis, DNA
Strongyloides ratti/drug effects/*genetics
Mutagenesis
Selection lines
Strongyloides ratti
Whole genome re-sequencing
Abstract:
Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing.
