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Free keywords:
Cell Respiration/genetics
Chaperonin 60/metabolism
Chaperonins/*chemistry
DNA, Mitochondrial/*genetics
Endopeptidase K/metabolism
Fungal Proteins/antagonists & inhibitors/biosynthesis/metabolism
Gene Deletion
*Gene Expression Regulation, Fungal
Heat-Shock Response/*genetics
Hot Temperature
Immune Sera
*Membrane Proteins
Mitochondria/chemistry/genetics/metabolism
Mitochondrial Proteins
Molecular Chaperones/chemistry/genetics/isolation & purification/*metabolism
Molecular Weight
Precipitin Tests
Protein Binding
Protein Biosynthesis/genetics
Ribosomal Proteins/antagonists & inhibitors/metabolism
Saccharomyces cerevisiae/cytology/*genetics
*Saccharomyces cerevisiae Proteins
Thermodynamics
Abstract:
Tcm62p, distantly related to chaperonins, is required for the assembly of succinate dehydrogenase in mitochondria of Saccharomyces cerevisiae and was proposed to exert chaperone activity. We demonstrate here crucial functions of Tcm62p under heat stress. It ensures mitochondrial gene expression at elevated temperatures and prevents heat-aggregation of the ribosomal subunit Var1p. Similar to chaperonins, Tcm62p forms a high molecular mass protein complex of approximately 850 kDa in the mitochondrial matrix space. These results suggest a more general chaperone function of Tcm62p in mitochondria.
