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  Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster

Menger, K. E., James, A. M., Cochemé, H. M., Harbour, M. E., Chouchani, E. T., Ding, S., et al. (2015). Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster. Cell Reports, 11(12), 1856-1865. doi:http://dx.doi.org/10.1016/j.celrep.2015.05.033.

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Menger, K. E., Author
James, A. M., Author
Cochemé, H. M., Author
Harbour, M. E., Author
Chouchani, E. T., Author
Ding, S., Author
Fearnley, I. M., Author
Partridge, L.1, Author           
Murphy, M. P., Author
Affiliations:
1Department Partridge - Biological Mechanisms of Ageing, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_1942287              

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 Abstract: Summary Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes in Drosophila melanogaster during aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo. Cysteine residues within young flies had a bimodal distribution with peaks at ∼10% and ∼85% reversibly oxidized. Surprisingly, these cysteine residues did not become more oxidized with age. In contrast, 24 hr of fasting dramatically oxidized cysteine residues that were reduced under fed conditions while also reducing cysteine residues that were initially oxidized. We conclude that fasting, but not aging, dramatically alters cysteine-residue redox status in D. melanogaster.

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 Dates: 2015-06-302015
 Publication Status: Issued
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 Identifiers: DOI: http://dx.doi.org/10.1016/j.celrep.2015.05.033
ISSN: 2211-1247
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Title: Cell Reports
Source Genre: Journal
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Pages: - Volume / Issue: 11 (12) Sequence Number: - Start / End Page: 1856 - 1865 Identifier: -