Molecular variability of FLT3/ITD mutants and their impact on the 
differentiation program of 32D cells: Implications for the biological 
properties of AML blasts

Pekova, S.; Ivanek, R.; Dvorak, M.; Rueggeberg , S.; Leicht, S.; Li, X. P.; Franz, T.; Kozak, T.; Vrba, J.; Koza, V.; Karas, M.; Schwarz, J.; Cetkovsky, P.; Prucha, M.

doi:10.1016/j.leukres.2009.01.004

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Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: Implications for the biological properties of AML blasts

Pekova, S., Ivanek, R., Dvorak, M., Rueggeberg, S., Leicht, S., Li, X. P., et al. (2009). Molecular variability of FLT3/ITD mutants and their impact on the differentiation program of 32D cells: Implications for the biological properties of AML blasts. Leukemia Research, 33(10), 1409-1416. doi:10.1016/j.leukres.2009.01.004.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000B-745A-E Version Permalink: https://hdl.handle.net/21.11116/0000-000B-745B-D

Genre: Journal Article

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Pekova, S., Author
Ivanek, R., Author
Dvorak, M., Author
Rueggeberg , S., Author
Leicht, S., Author
Li, X. P., Author
Franz, T.¹, Author
Kozak, T., Author
Vrba, J., Author
Koza, V., Author
Karas, M., Author
Schwarz, J., Author
Cetkovsky, P., Author
Prucha, M., Author

Affiliations:
1Proteomics, Core Facilities, Max Planck Institute for Biology of Ageing, Max Planck Society, ou_1942305

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Free keywords: flt3/itd hematopoiesis aml microarrays proteomics acute myeloid-leukemia internal tandem duplication hematopoietic stem-cell expression mutations receptor survival ligand electrophoresis malignancies

Abstract: FLT3 is the most frequently mutated gene in acute myeloid leukemia (AML), with internal tandem duplications (ITDs) accounting for up to 30% of its mutations. To analyze the impact of individual ITDs on the expression profile of immature myeloid cells, we have established 32D cell lines expressing nine different FLT3/ITDs isolated from AML patients and subjected them to whole genome expression profiling and 2DE/LC/MS proteomics. Our data indicate that in comparison to the controls, FLT3/ITD-positive 32D cells exhibit less mature expression profiles resembling early hematopoietic progenitors. Moreover, our results suggest that there exist biological differences among individual ITD variants. (c) 2009 Elsevier Ltd. All rights reserved.

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Language(s): eng - English

Dates: Published Online: 2009-10Date issued: 2009

Publication Status: Issued

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Identifiers: Other: WOS:000268375500023
DOI: 10.1016/j.leukres.2009.01.004
ISSN: 0145-2126

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Title: Leukemia Research

Alternative Title : Leukemia Res

Source Genre: Journal

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Pages: - Volume / Issue: 33 (10) Sequence Number: - Start / End Page: 1409 - 1416 Identifier: -