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  A leader cell triggers end of lag phase in populations of Pseudomonas fluorescens

Ardré, M., Doulcier, G., Brenner, N., & Rainey, P. B. (2022). A leader cell triggers end of lag phase in populations of Pseudomonas fluorescens. microLife, 3: uqac022. doi:10.1093/femsml/uqac022.

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uqac022.pdf (Verlagsversion), 2MB
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2022
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© The Author(s) 2022

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 Urheber:
Ardré, Maxime, Autor
Doulcier, Guilhem, Autor
Brenner, Naama, Autor
Rainey, Paul B.1, Autor                 
Affiliations:
1Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2421699              

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Schlagwörter: High-throughput millifluidics, Growth dynamics, Collective behaviour, Extreme Value Theory, Microbialpopulation biology
 Zusammenfassung: The relationship between the number of cells colonizing a new environment and time for resumption of growth is a subject of long-standing interest. In microbiology this is known as the ‘inoculum effect’. Its mechanistic basis is unclear with possible explanations ranging from the independent actions of individual cells, to collective actions of populations of cells. Here we use a millifluidic droplet device in which the growth dynamics of hundreds of populations founded by controlled numbers of Pseudomonas fluorescens cells, ranging from a single cell, to one thousand cells, were followed in real time. Our data show that lag phase decreases with inoculum size. The average decrease, variance across droplets, and distribution shapes, follow predictions of extreme value theory, where the inoculum lag time is determined by the minimum value sampled from the single-cell distribution. Our experimental results show that exit from lag phase depends on strong interactions among cells, consistent with a ‘leader cell’ triggering end of lag phase for the entire population.

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Sprache(n): eng - English
 Datum: 2022-10-132022-10-252022-11-022022-12-01
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1093/femsml/uqac022
 Art des Abschluß: -

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Titel: microLife
  Kurztitel : microLife
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Oxford, UK : Oxford University Press
Seiten: - Band / Heft: 3 Artikelnummer: uqac022 Start- / Endseite: - Identifikator: ISSN: 2633-6693
CoNE: https://pure.mpg.de/cone/journals/resource/2633-6693