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  From the Microbiome to the Gene: Mapping the genes in a leaf microbiome responsible for strain-specific pathogenicity

Karasov, T., Neumann, M., Duque, A., Ashkenazy, H., & Weigel, D. (2019). From the Microbiome to the Gene: Mapping the genes in a leaf microbiome responsible for strain-specific pathogenicity. Molecular Plant-Microbe Interactions, 32(10S), S1.238.

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 Creators:
Karasov, T1, Author                 
Neumann, M1, Author                 
Duque, A1, Author           
Ashkenazy, H1, Author                 
Weigel, D1, Author                 
Affiliations:
1Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375790              

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 Abstract: Microbiome sequencing provides a snapshot of the composition of a microbial community. Determining which genes and functions enable specific taxa to proliferate within a microbial community remains a central challenge. In this study, we aim to determine the genes that increase the proliferation of specific Pseudomonas in the microbiomes of wild Arabidopsis thaliana populations. We recently found that the most
abundant Pseudomonas taxon in A. thaliana in Southwestern Germany was composed of genotypically and phenotypically diverse strains. Closely related strains—indistinguishable via 16S amplicon sequencing—show a range of pathogenicity phenotypes. Here we combine microbiome analysis, whole genome sequencing of strains, genome-wide association mapping and saturation screening to identify those genes important for strain success. The frequent gain and loss of these genes underscores the capacity for rapid evolution in Pseudomonas.

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 Dates: 2019-10
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1094/MPMI-32-10-S1.213
 Degree: -

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Title: XVIII Congress of the International Society for Molecular Plant-Microbe Interactions (IS-MPMI 2019)
Place of Event: Glasgow, UK
Start-/End Date: 2019-07-14 - 2019-07-18

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Title: Molecular Plant-Microbe Interactions
Source Genre: Journal
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Publ. Info: St. Paul, MN, USA : APS Press
Pages: - Volume / Issue: 32 (10S) Sequence Number: - Start / End Page: S1.238 Identifier: ISSN: 0894-0282
CoNE: https://pure.mpg.de/cone/journals/resource/954925558505