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  E-box function in a period gene repressed by light

Vallone, D., Gondi, S., Whitmore, D., & Foulkes, N. (2004). E-box function in a period gene repressed by light. Proceedings of the National Academy of Sciences of the United States of America, 101(12), 4106-4111.

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Vallone, D1, Author                 
Gondi, SB1, Author           
Whitmore, D, Author
Foulkes, NS1, Author                 
Affiliations:
1Research Group Zebrafish Chronobiology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3391226              

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 Abstract: In most organisms, light plays a key role in the synchronization of the circadian timing system with the environmental day-night cycle. Light pulses that phase-shift the circadian clock also induce the expression of period (per) genes in vertebrates. Here, we report the cloning of a zebrafish per gene, zfper4, which is remarkable in being repressed by light. We have developed an in vivo luciferase reporter assay for this gene in cells that contain a light-entrainable clock. High-definition bioluminescence traces have enabled us to accurately measure phase-shifting of the clock by light. We have also exploited this model to study how four E-box elements in the zfper4 promoter regulate expression. Mutagenesis reveals that the integrity of these four E-boxes is crucial for maintaining low basal expression together with robust rhythmicity and repression by light. Importantly, in the context of a minimal heterologous promoter, the E-box elements also direct a robust circadian rhythm of expression that is significantly phase-advanced compared with the original zfper4 promoter and lacks the light-repression property. Thus, these results reveal flexibility in the phase and light responsiveness of E-box-directed rhythmic expression, depending on the promoter context.

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 Dates: 2004-03
 Publication Status: Published in print
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 101 (12) Sequence Number: - Start / End Page: 4106 - 4111 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230