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  Predicting the Success of Fmoc-Based Peptide Synthesis

Gutman, I., Gutman, R., Sidney, J., Chihab, L., Mishto, M., Liepe, J., et al. (2022). Predicting the Success of Fmoc-Based Peptide Synthesis. ACS Omega, 7, 23771-23781. doi:10.1021/acsomega.2c02425.

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 Urheber:
Gutman, Ilanit, Autor
Gutman, Ron, Autor
Sidney, John, Autor
Chihab, Leila, Autor
Mishto, Michele, Autor
Liepe, Juliane1, Autor           
Chiem, Anthony, Autor
Greenbaum, Jason, Autor
Yan, Zhen, Autor
Sette, Alessandro, Autor
Koşaloğlu-Yalçın, Zeynep, Autor
Peters, Bjoern, Autor
Affiliations:
1Research Group of Quantitative and Systems Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350287              

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 Zusammenfassung: Synthetic peptides are commonly used in biomedical science for many applications in basic and translational research. While peptide synthesis is generally easy and reliable, the chemical nature of some amino acids as well as the many steps and chemical compounds involved can render the synthesis of some peptide sequences difficult. Identification of these problematic sequences and mitigation of issues they may present can be important for the reliable use of peptide reagents in several contexts. Here, we assembled a large dataset of peptides that were synthesized using standard Fmoc chemistry and whose identity was validated using mass spectrometry. We analyzed the mass spectra to identify errors in peptide syntheses and sought to develop a computational tool to predict the likelihood that any given peptide sequence would be synthesized accurately. Our model, named Peptide Synthesis Score (PepSySco), is able to predict the likelihood that a peptide will be successfully synthesized based on its amino acid sequence.

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Sprache(n): eng - English
 Datum: 2022-06-27
 Publikationsstatus: Online veröffentlicht
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1021/acsomega.2c02425
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Projektname : Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health under award number U24CA248138 and by the National Institute of Allergy and Infectious Diseases (NIAID) under award number 75N93019C00001. M.M. and J.L. were in part supported by (i) Cancer Research UK [C67500; A29686] and the National Institute for Health Research (NIHR) Biomedical Research Centre based at Guy’s and St Thomas’ NHS Foundation Trust and King’s College London and/or the NIHR Clinical Research Facility to M.M. and (ii) the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no 945528) to J.L. We thank S. Lyham and X. Yang (KCL) for technical assistance.
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Förderprogramm : -
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Projektname : IMAP
Grant ID : 945528
Förderprogramm : Horizon 2020 (H2020)
Förderorganisation : European Commission (EC)

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Titel: ACS Omega
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, DC : American Chemical Society
Seiten: - Band / Heft: 7 Artikelnummer: - Start- / Endseite: 23771 - 23781 Identifikator: ISSN: 2470-1343
CoNE: https://pure.mpg.de/cone/journals/resource/2470-1343