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  Mussels fabricate porous glues via multiphase liquid–liquid phase separation of multiprotein condensates

Renner-Rao, M., Jehle, F., Priemel, T., Duthoo, E., Fratzl, P., Bertinetti, L., et al. (2022). Mussels fabricate porous glues via multiphase liquid–liquid phase separation of multiprotein condensates. ACS Nano, 16(12), 20877-20890. doi:10.1021/acsnano.2c08410.

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Renner-Rao, Max, Autor
Jehle, Franziska1, Autor           
Priemel, Tobias, Autor
Duthoo, Emilie, Autor
Fratzl, Peter2, Autor                 
Bertinetti, Luca3, Autor                 
Harrington, Matthew J., Autor
Affiliations:
1Franziska Jehle, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_3389297              
2Peter Fratzl, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863294              
3Luca Bertinetti, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2379691              

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Schlagwörter: protein condensates; liquid-liquid phase separation (LLPS); mussel byssus; adhesion; self-assembly; coacervation
 Zusammenfassung: Mussels (Mytilus edulis) adhere to hard surfaces in intertidal marine habitats with a porous underwater glue called the byssus plaque. The plaque is an established role model for bioinspired underwater glues and comprises at least six proteins, most of which are highly cationic and enriched in the post-translationally modified amino acid 3,4-dihydroxyphenylalanine (DOPA). While much is known about the chemistry of plaque adhesion, less is understood about the natural plaque formation process. Here, we investigated plaque structure and formation using 3D electron microscopic imaging, revealing that micro- and nanopores form spontaneously during secretion of protein-filled secretory vesicles. To better understand this process, we developed a method to purify intact secretory vesicles for in vitro assembly studies. We discovered that each vesicle contains a sulfate-associated fluid condensate consisting of ∼9 histidine- and/or DOPA-rich proteins, which are presumably the required ingredients for building a plaque. Rupturing vesicles under specific buffering conditions relevant for natural assembly led to controlled multiphase liquid–liquid phase separation (LLPS) of different proteins, resulting in formation of a continuous phase with coexisting droplets. Rapid coarsening of the droplet phase was arrested through pH-dependent cross-linking of the continuous phase, producing native-like solid porous “microplaques” with droplet proteins remaining as fluid condensates within the pores. Results indicate that histidine deprotonation and sulfates figure prominently in condensate cross-linking. Distilled concepts suggest that combining phase separation with tunable cross-linking kinetics could be effective for microfabricating hierarchically porous materials via self-assembly.

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Sprache(n): eng - English
 Datum: 2022-11-222022
 Publikationsstatus: Erschienen
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 Identifikatoren: DOI: 10.1021/acsnano.2c08410
PMID: 0634
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Titel: ACS Nano
  Kurztitel : ACS Nano
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, DC : American Chemical Society
Seiten: - Band / Heft: 16 (12) Artikelnummer: - Start- / Endseite: 20877 - 20890 Identifikator: ISSN: 1936-0851