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Free keywords:
ACYL CARRIER PROTEIN; MULTIENZYME COMPLEX; STRUCTURAL BASIS;
FORCE-FIELD; YEAST; SPECIFICITY; SUBSTRATE; BIOSYNTHESIS; INHIBITION;
SYNTHETASEBiochemistry & Molecular Biology;
Abstract:
In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds.
