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  Fibronectin anchoring to viscoelastic poly(dimethylsiloxane) elastomers controls fibroblast mechanosensing and directional motility

Missirlis, D., Heckmann, L., Haraszti, T., & Spatz, J. P. (2022). Fibronectin anchoring to viscoelastic poly(dimethylsiloxane) elastomers controls fibroblast mechanosensing and directional motility. Biomaterials, 287: 121646, pp. 1-11. doi:10.1016/j.biomaterials.2022.121646.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000B-A75A-4 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-A75B-3
Genre: Journal Article

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 Creators:
Missirlis, Dimitris1, Author           
Heckmann, Lara1, Author           
Haraszti, Tamás, Author
Spatz, Joachim P.1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: The established link between deregulated tissue mechanics and various pathological states calls for the elucidation of the processes through which cells interrogate and interpret the mechanical properties of their microenvironment. In this work, we demonstrate that changes in the presentation of the extracellular matrix protein fibronectin on the surface of viscoelastic silicone elastomers have an overarching effect on cell mechanosensing, that is independent of bulk mechanics. Reduction of surface hydrophilicity resulted in altered fibronectin adsorption strength as monitored using atomic force microscopy imaging and pulling experiments. Consequently, primary human fibroblasts were able to remodel the fibronectin coating, adopt a polarized phenotype and migrate directionally even on soft elastomers, that otherwise were not able to resist the applied traction forces. The findings presented here provide valuable insight on how cellular forces are regulated by ligand presentation and used by cells to probe their mechanical environment, and have implications on biomaterial design for cell guidance.

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Language(s): eng - English
 Dates: 2022-05-242022-01-252022-06-222022-06-272022-08
 Publication Status: Issued
 Pages: 11
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.biomaterials.2022.121646
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Title: Biomaterials
Source Genre: Journal
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Publ. Info: Guildford, England : Elsevier
Pages: - Volume / Issue: 287 Sequence Number: 121646 Start / End Page: 1 - 11 Identifier: ISSN: 0142-9612
CoNE: https://pure.mpg.de/cone/journals/resource/954925472369