English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Functional specificity of the nematode Hox gene mab-5

Gutierrez, A., Knoch, L., Witte, H., & Sommer, R. (2003). Functional specificity of the nematode Hox gene mab-5. Development, 130(5), 983-993. doi:10.1242/dev.00320.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Gutierrez, A1, Author           
Knoch, L1, Author           
Witte, H1, Author                 
Sommer, RJ1, Author                 
Affiliations:
1Department Integrative Evolutionary Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375786              

Content

show
hide
Free keywords: -
 Abstract: Hox genes encode evolutionarily conserved transcription factors involved in morphological specification along the anteroposterior body axis of animals. The two most striking features of Hox genes are colinearity and the strong sequence conservation. Among all animals studied so far, the nematode Caenorhabditis elegans contains one of the most divergent Hox clusters. The core cluster contains only four members, which in part deviate from the colinearity rule. In addition, orthologous and paralogous nematode Hox sequences diverged substantially. Given these nematode-specific features, we asked how these Hox proteins evolved and how they provide functional specificity. We investigated the role of MAB-5 during ray formation and established an in vivo assay using Cel-mab-5 regulatory elements to express orthologous, paralogous and chimeric cDNAs in a Cel-mab-5 mutant background. We show that the MAB-5 ortholog from Pristionchus pacificus, but not the C. elegans paralogous Hox proteins can rescue Cel-mab-5. Experiments with chimeric, truncated and mutagenized Hox proteins suggest the specificity to be conferred by the N-terminal arm and helix I, but not helix II of the homeodomain.

Details

show
hide
Language(s):
 Dates: 2003-03
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1242/dev.00320
PMID: 12538523
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Development
  Other : Development
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Cambridgeshire : Company of Biologists
Pages: - Volume / Issue: 130 (5) Sequence Number: - Start / End Page: 983 - 993 Identifier: ISSN: 0950-1991
CoNE: https://pure.mpg.de/cone/journals/resource/954927546241