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  Screening for eukaryotic motifs in Legionella pneumophila reveals Smh1 as bacterial deacetylase of host histones

Herbel, S. M., Moyon, L., Christ, M., Elsayed, E. M., Caffrey, B. E., Malmsheimer, S., et al. (2022). Screening for eukaryotic motifs in Legionella pneumophila reveals Smh1 as bacterial deacetylase of host histones. Virulence, 13(1), 2042-2058. doi:10.1080/21505594.2022.2149973.

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https://doi.org/10.1080/21505594.2022.2149973 (Verlagsversion)
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 Urheber:
Herbel, S. M., Autor
Moyon, L., Autor
Christ, M., Autor
Elsayed, E. M., Autor
Caffrey, B. E., Autor
Malmsheimer, S., Autor
Grin, I., Autor
Hoffmann, K., Autor
Surmann, K., Autor
Blankenburg, S., Autor
Jung, A. L., Autor
Herkt, C. E., Autor
Borsò, M., Autor
Bozdag, B., Autor
Imhof, A., Autor
Becker, A., Autor
Wagner, S., Autor
Bange, G.1, 2, 3, Autor                 
Völker, U., Autor
Bertrams, W., Autor
Marsico, A., AutorSchmeck, B., Autor mehr..
Affiliations:
1Max Planck Fellow Molecular Physiology of Microbes, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3321791              
2Philipps-Universität Marburg, Department Chemistry, ou_persistent22              
3Philipps-Universität Marburg, Center for Synthetic Microbiology, ou_persistent22              

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Schlagwörter: Humans *Eukaryota Histones/genetics *Legionella pneumophila/genetics Eukaryotic Cells Research Virulence Factors/genetics Histone Deacetylases Repressor Proteins Infection Legionella pneumophila Smh1 histone-deacetylase macrophage
 Zusammenfassung: Legionella pneumophila (L.p.) is a bacterial pathogen which is a common causative agent of pneumonia. In humans, it infects alveolar macrophages and transfers hundreds of virulence factors that interfere with cellular signalling pathways and the transcriptomic landscape to sustain its own replication. By this interaction, it has acquired eukaryote-like protein motifs by gene transfer events that partake in the pathogenicity of Legionella. In a computational screening approach for eukaryotic motifs in the transcriptome of Legionella, we identified the L.p. strain Corby protein ABQ55614 as putative histone-deacetylase and named it "suppressing modifier of histones 1" (Smh1). During infection, Smh1 is translocated from the Legionella vacuole into the host cytosol. When expressed in human macrophage THP-1 cells, Smh1 was localized predominantly in the nucleus, leading to broad histone H3 and H4 deacetylation, blunted expression of a large number of genes (e.g. IL-1β and IL-8), and fostered intracellular bacterial replication. L.p. with a Smh1 knockdown grew normally in media but showed a slight growth defect inside the host cell. Furthermore, Smh1 showed a very potent histone deacetylation activity in vitro, e.g. at H3K14, that could be inhibited by targeted mutation of the putative catalytic center inferred by analogy with eukaryotic HDAC8, and with the deacetylase inhibitor trichostatin A. In summary, Smh1 displays functional homology with class I/II type HDACs. We identified Smh1 as a new Legionella virulence factor with a eukaryote-like histone-deacetylase activity that moderates host gene expression and might pave the way for further histone modifications.IMPORTANCELegionella pneumophila (L.p.) is a prominent bacterial pathogen, which is a common causative agent of pneumonia. In order to survive inside the host cell, the human macrophage, it profoundly interacts with host cell processes to advance its own replication. In this study, we identify a bacterial factor, Smh1, with yet unknown function as a host histone deacetylase. The activity of this factor in the host cell leads to attenuated gene expression and increased intracellular bacterial replication.

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Sprache(n): eng - English
 Datum: 2022-11-22
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: Anderer: 36411449
DOI: 10.1080/21505594.2022.2149973
ISSN: 2150-5594 (Print)2150-5594
 Art des Abschluß: -

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Titel: Virulence
Genre der Quelle: Zeitschrift
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Affiliations:
Ort, Verlag, Ausgabe: Landes Bioscience
Seiten: - Band / Heft: 13 (1) Artikelnummer: - Start- / Endseite: 2042 - 2058 Identifikator: ISSN: 2150-5608
CoNE: https://pure.mpg.de/cone/journals/resource/2150-5608