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  Structures of the eukaryotic ribosome and its translational states in situ

Hoffmann, P. C., Kreysing, J. P., Khusainov, I., Tuijtel, M., Welsch, S., & Beck, M. (2022). Structures of the eukaryotic ribosome and its translational states in situ. Nature Communications, 13: 7435. doi:10.1038/s41467-022-34997-w.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000B-B2D0-0 Version Permalink: https://hdl.handle.net/21.11116/0000-000E-4CBB-B
Genre: Journal Article

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 Creators:
Hoffmann, Patrick C.1, Author                 
Kreysing, Jan Philipp1, 2, Author                 
Khusainov, Iskander1, Author                 
Tuijtel, Maarten1, Author                 
Welsch, Sonja3, Author                 
Beck, Martin1, Author                 
Affiliations:
1Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society, ou_3040395              
2IMPRS-CBP, Max Planck Institute of Biophysics, Max Planck Society, ou_3562496              
3Central Electron Microscopy Facility, Max Planck Institute of Biophysics, Max Planck Society, ou_3249263              

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 Abstract: Ribosomes translate genetic information into primary structure. During translation, various cofactors transiently bind to the ribosome that undergoes prominent conformational and structural changes. Different translational states of ribosomes have been well characterized in vitro. However, to which extent the known translational states are representative of the native situation inside cells has thus far only been addressed in prokaryotes. Here, we apply cryo-electron tomography to cryo-FIB milled Dictyostelium discoideum cells combined with subtomogram averaging and classification. We obtain an in situ structure that is locally resolved up to 3 Angstrom, the distribution of eukaryotic ribosome translational states, and unique arrangement of rRNA expansion segments. Our work demonstrates the use of in situ structural biology techniques for identifying distinct ribosome states within the cellular environment.

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Language(s): eng - English
 Dates: 2022-06-032022-11-152022-12-02
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-022-34997-w
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 13 Sequence Number: 7435 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723