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  A Clathrin light chain A reporter mouse for in vivo imaging of endocytosis

Grimm, E., van der Hoeven, F., Sardella, D., Willig, K. I., Engel, U., Nisha, V., et al. (2022). A Clathrin light chain A reporter mouse for in vivo imaging of endocytosis. PLoS One, 17(9): 0273660, pp. 1-24. doi:10.1371/journal.pone.0273660.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000B-B647-8 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-B648-7
Genre: Journal Article

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 Creators:
Grimm, Elisabeth, Author
van der Hoeven, Franciscus, Author
Sardella, Donato, Author
Willig, Katrin I., Author
Engel, Ulrike, Author
Nisha, Veits1, Author           
Engel, Robert, Author
Cavalcanti-Adam, Elisabetta Ada1, Author           
Bestvater, Felix, Author
Bordoni, Luca, Author
Jennemann, Richard, Author
Schönig, Kai, Author
Schiessl, Ina Maria, Author
Sandhoff, Roger, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: Clathrin-mediated endocytosis (CME) is one of the best studied cellular uptake pathways and its contributions to nutrient uptake, receptor signaling, and maintenance of the lipid membrane homeostasis have been already elucidated. Today, we still have a lack of understanding how the different components of this pathway cooperate dynamically in vivo. Therefore, we generated a reporter mouse model for CME by fusing eGFP endogenously in frame to clathrin light chain a (Clta) to track endocytosis in living mice. The fusion protein is expressed in all tissues, but in a cell specific manner, and can be visualized using fluorescence microscopy. Recruitment to nanobeads recorded by TIRF microscopy validated the functionality of the Clta-eGFP reporter. With this reporter model we were able to track the dynamics of Alexa594-BSA uptake in kidneys of anesthetized mice using intravital 2-photon microscopy. This reporter mouse model is not only a suitable and powerful tool to track CME in vivo in genetic or disease mouse models it can also help to shed light into the differential roles of the two clathrin light chain isoforms in health and disease.

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Language(s): eng - English
 Dates: 2021-12-162022-08-132022-09-23
 Publication Status: Published online
 Pages: 24
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1371/journal.pone.0273660
URI: https://pubmed.ncbi.nlm.nih.gov/36149863/
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Title: PLoS One
  Abbreviation : PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 17 (9) Sequence Number: 0273660 Start / End Page: 1 - 24 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850