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  Antibodies against the T61 antigen inhibit neuronal migration in the chick optic tectum

Meyer, S., & Henke-Fahle, S. (1995). Antibodies against the T61 antigen inhibit neuronal migration in the chick optic tectum. Proceedings of the National Academy of Sciences of the United States of America, 92(24), 11150-11154. doi:10.1073/pnas.92.24.11150.

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Meyer, SU1, Author           
Henke-Fahle, S1, Author           
Affiliations:
1Department Physical Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3384683              

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 Abstract: Cell migration in the central nervous system depends, in part, on receptors and extracellular matrix molecules that likewise support axonal outgrowth. We have investigated the influence of T61, a monoclonal antibody that has been shown to inhibit growth cone motility in vitro, on neuronal migration in the developing optic tectum. Intraventricular injections of antibody-producing hybridoma cells or ascites fluid were used to determine the action of this antibody in an in vivo environment. To document alterations in tectal layer formation, a combination of cell-nuclei staining and axonal immunolabeling methods was employed. In the presence of T61 antibody, cells normally destined for superficial layers accumulated in the ventricular zone instead, leading to a reduction of the cell-dense layer in the tectal plate. Experiments with 5-bromo-2'-deoxyuridine labeling followed by antibody staining confirmed that the nonmigrating cells remaining in the ventricular zone were postmitotic and had differentiated. The structure of radial glial cells, as judged by staining with a glia-specific antibody and the fluorescent tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI), remained intact in these embryos. Our findings suggest that the T61 epitope is involved in a mechanism underlying axonal extension and neuronal migration, possibly by influencing the motility of the leading process.

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 Dates: 1995-11
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.1073/pnas.92.24.11150
PMID: 7479955
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 92 (24) Sequence Number: - Start / End Page: 11150 - 11154 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230