Cell Surface Engineering Enables Surfaceome Profiling

Vilen, Zak; Reeves, Abigail E.; O’Leary, Timothy R.; Joeh, Eugene; Kamasawa, Naomi; Huang, Mia L.

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Cell Surface Engineering Enables Surfaceome Profiling

Vilen, Z., Reeves, A. E., O’Leary, T. R., Joeh, E., Kamasawa, N., & Huang, M. L. (2022). Cell Surface Engineering Enables Surfaceome Profiling. ACS Chemical Biology. Retrieved from https://pubs.acs.org/doi/pdf/10.1021/acschembio.1c00865.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-000B-FBF1-A Versions-Permalink: https://hdl.handle.net/21.11116/0000-000B-FBF2-9

Genre: Zeitschriftenartikel

Alternativer Titel : ACS Chemical Biology

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Urheber:
Vilen, Zak, Autor
Reeves, Abigail E., Autor
O’Leary, Timothy R., Autor
Joeh, Eugene, Autor
Kamasawa, Naomi¹, Autor
Huang, Mia L., Autor

Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288

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Zusammenfassung: Cell surface proteins (CSPs) are vital molecular mediators for cells and their extracellular environment. Thus, understanding which CSPs are displayed on cells, especially in different cell states, remains an important endeavor in cell biology. Here, we describe the integration of cell surface engineering with radical-mediated protein biotinylation to profile CSPs. This method relies on the prefunctionalization of cells with cholesterol lipid groups, followed by sortase-catalyzed conjugation with an APEX2 ascorbate peroxidase enzyme. In the presence of biotin-phenol and H2O2, APEX2 catalyzes the formation of highly reactive biotinyl radicals that covalently tag electron-rich residues within CSPs for subsequent streptavidin-based enrichment and analysis by quantitative mass spectrometry. While APEX2 is traditionally used to capture proximity-based interactomes, we envisioned using it in a “baitless” manner on cell surfaces to capture CSPs. We evaluate this strategy in light of another CSP labeling method that relies on the presence of cell surface sialic acid. Using the APEX2 strategy, we describe the CSPs found in three mammalian cell lines and compare CSPs in adherent versus three-dimensional pancreatic adenocarcinoma cells.