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  RNA-Dependent Cysteine Biosynthesis in Archaea

Yuan, J., Sauerwald, A., Zhu, W., Major, T., Roy, H., Palioura, S., et al. (2006). RNA-Dependent Cysteine Biosynthesis in Archaea. The FASEB Journal, 20(4), A503-A504. Retrieved from https://doi.org/10.1096/fasebj.20.4.A503-d.

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Genre: Journal Article
Alternative Title : The FASEB Journal

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 Creators:
Yuan, Jing1, Author                 
Sauerwald, Anselm2, Author
Zhu, Wenhong2, Author
Major, Tiffany2, Author
Roy, Hervé2, Author
Palioura, Sotiria2, Author
Jahn, Dieter2, Author
Whitman, William2, Author
Yates III, John2, Author
Ibba, Michael2, Author
Söll, Dieter2, Author
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1Department of Molecular Biophysics and Biochemistry, Yale University, USA, ou_persistent22              
2external, ou_persistent22              

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 Abstract: Several methanogenic archaea lack cysS the gene encoding cysteinyl-tRNA synthetase (CysRS), the essential enzyme that provides Cys-tRNACys for translation in most organisms. Using anaerobic techniques to fractionate and analyze the cell-extract of Methanocaldococcus jannaschii revealed that tRNACys becomes acylated with O-phosphoserine (Sep) by a class II-type O-phosphoseryl-tRNA synthetase. Sep-tRNACys is then converted to Cys-tRNACys by Sep-tRNA:Cys-tRNA synthase (SepCysS) in the presence of an unknown sulfur donor. Genomic analyses revealed that this pathway is found in all organisms lacking CysRS as well as some other archaea that contain cysS. Genetic experiments demonstrated that this pathway is also the sole route for tRNA-dependent cysteine biosynthesis in some methanogens. The presence of Sep-tRNASec was established recently (Kaiser et al, Biochemistry 2005). As the conversions of Sep-tRNA to Cys-tRNA or Sec-tRNA are chemically analogous, the catalytic activity of SepCysS or of a similar enzyme may have provided the means by which selenocysteine evolved and was added to the genetic code. Experiments along these lines are currently in progress. (This work was supported by grants from NIGMS, National Institutes of Health, and the U. S. Department of Energy).

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 Dates: 2006-03
 Publication Status: Issued
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Title: The FASEB Journal
  Alternative Title : The FASEB Journal
Source Genre: Journal
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Publ. Info: John Wiley & Sons, Ltd
Pages: - Volume / Issue: 20 (4) Sequence Number: - Start / End Page: A503 - A504 Identifier: ISBN: 0892-6638

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Title: The FASEB Journal
  Alternative Title : The FASEB Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: John Wiley & Sons, Ltd
Pages: - Volume / Issue: 20 (4) Sequence Number: - Start / End Page: A503 - A504 Identifier: ISBN: 0892-6638

Source 3

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Title: The FASEB Journal
  Alternative Title : The FASEB Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: John Wiley & Sons, Ltd
Pages: - Volume / Issue: 20 (4) Sequence Number: - Start / End Page: A503 - A504 Identifier: ISBN: 0892-6638