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  Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

Yang, L., Machin, F., Wang, S., Saplaoura, E., & Kragler, F. (2023). Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks. Nature Biotechnology, 41, 958-967. doi:10.1038/s41587-022-01585-8.

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 Creators:
Yang, L.1, Author           
Machin, F.1, Author           
Wang, S.1, Author           
Saplaoura, E.1, Author           
Kragler, F.1, Author           
Affiliations:
1Intercellular Macromolecular Transport, Department Köhler, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_3338335              

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 Abstract: Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce transgene-free lines. We have addressed this issue by designing fusions of Cas9 and guide RNA transcripts to tRNA-like sequence motifs that move RNAs from transgenic rootstocks to grafted wild-type shoots (scions) and achieve heritable gene editing, as demonstrated in wild-type Arabidopsis thaliana and Brassica rapa. The graft-mobile gene editing system enables the production of transgene-free offspring in one generation without the need for transgene elimination, culture recovery and selection, or use of viral editing vectors. We anticipate that using graft-mobile editing systems for transgene-free plant production may be applied to a wide range of breeding programs and crop plants.

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Language(s): eng - English
 Dates: 2023-01-022023
 Publication Status: Issued
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 Identifiers: DOI: 10.1038/s41587-022-01585-8
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Title: Nature Biotechnology
Source Genre: Journal
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Pages: - Volume / Issue: 41 Sequence Number: - Start / End Page: 958 - 967 Identifier: ISBN: 1546-1696