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  Extracellular cues govern shape and cytoskeletal organization in giant unilamellar lipid vesicles

Fink, A., Doll, C. R., Relimpio, A. Y., Dreher, Y., Spatz, J. P., Göpfrich, K., et al. (2023). Extracellular cues govern shape and cytoskeletal organization in giant unilamellar lipid vesicles. ACS Synthetic Biology, 12(2), 369-374. doi:10.1021/acssynbio.2c00516.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-6CB1-3 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-B1C0-2
Genre: Journal Article

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 Creators:
Fink, Andreas1, Author           
Doll, Charlotte R.1, Author           
Relimpio, Ana Yagüe1, Author           
Dreher, Yannik1, Author           
Spatz, Joachim P.1, Author           
Göpfrich, Kerstin1, Author           
Cavalcanti-Adam, Elisabetta Ada1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: Spontaneous and induced front-rear polarization and a subsequent asymmetric actin cytoskeleton is a crucial event leading to cell migration, a key process involved in a variety of physiological and pathological conditions such as tissue development, wound healing, and cancer. Migration of adherent cells relies on the balance between adhesion to the underlying matrix and cytoskeleton-driven front protrusion and rear retraction. A current challenge is to uncouple the effect of adhesion and shape from the contribution of the cytoskeleton in regulating the onset of front-rear polarization. Here, we present a minimal model system that introduces an asymmetric actin cytoskeleton in synthetic cells, which are resembled by giant unilamellar lipid vesicles (GUVs) adhering onto symmetric and asymmetric micropatterned surfaces. Surface micropatterning of streptavidin-coated regions with varying adhesion shape and area was achieved by maskless UV photopatterning. To further study the effects of GUV shape on the cytoskeletal organization, actin filaments were polymerized together with bundling proteins inside the GUVs. The micropatterns induce synthetic cell deformation upon adhesion to the surface, with the cell shape adapting to the pattern shape and size. As expected, asymmetric patterns induce an asymmetric deformation in adherent synthetic cells. Actin filaments orient along the long axis of the deformed GUV, when having a length similar to the size of the major axis, whereas short filaments exhibit random orientation. With this bottom-up approach we have laid the first steps to identify the relationship between cell front-rear polarization and cytoskeleton organization in the future. Such a minimal system will allow us to further study the major components needed to create a polarized cytoskeleton at the onset of migration.

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Language(s): eng - English
 Dates: 2022-09-282023-01-182023-02-17
 Publication Status: Issued
 Pages: 6
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 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acssynbio.2c00516
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Title: ACS Synthetic Biology
  Abbreviation : ACS Synth. Biol.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 12 (2) Sequence Number: - Start / End Page: 369 - 374 Identifier: ISSN: 2161-5063
CoNE: https://pure.mpg.de/cone/journals/resource/2161-5063