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  Reconstitution of yeast microsomal lipid flip-flop using endogenous aminophospholipids

Nicolson, T., & Mayinger, P. (2000). Reconstitution of yeast microsomal lipid flip-flop using endogenous aminophospholipids. FEBS Letters, 476(3), 277-281. doi:10.1016/s0014-5793(00)01684-7.

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Nicolson, T1, Author                 
Mayinger, P, Author
Affiliations:
1Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375716              

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 Abstract: The molecular basis of transbilayer movement or flipping of phospholipids in the endoplasmic reticulum is largely unknown. To circumvent the problems inherent to studies with artificial phospholipid analogs, we studied microsomal flip-flop of endogenous phosphatidylethanolamine in yeast. The transbilayer transport of phosphatidylethanolamine was measured in reconstituted proteoliposomes derived from microsomal detergent extracts. Our results demonstrate that flipping is protease sensitive but does not require metabolic energy. Our assay is the first to use the endogenous substrate of the so-called 'flippase' to study phospholipid translocation in endomembranes and may therefore be crucial for the understanding of the catalytic properties of this elusive enzyme.

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 Dates: 2000-07
 Publication Status: Issued
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 Identifiers: DOI: 10.1016/s0014-5793(00)01684-7
PMID: 10913628
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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 476 (3) Sequence Number: - Start / End Page: 277 - 281 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501