English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Reconstitution of yeast microsomal lipid flip-flop using endogenous aminophospholipids

Nicolson, T., & Mayinger, P. (2000). Reconstitution of yeast microsomal lipid flip-flop using endogenous aminophospholipids. FEBS Letters, 476(3), 277-281. doi:10.1016/s0014-5793(00)01684-7.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Nicolson, T1, Author                 
Mayinger, P, Author
Affiliations:
1Department Genetics, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375716              

Content

show
hide
Free keywords: -
 Abstract: The molecular basis of transbilayer movement or flipping of phospholipids in the endoplasmic reticulum is largely unknown. To circumvent the problems inherent to studies with artificial phospholipid analogs, we studied microsomal flip-flop of endogenous phosphatidylethanolamine in yeast. The transbilayer transport of phosphatidylethanolamine was measured in reconstituted proteoliposomes derived from microsomal detergent extracts. Our results demonstrate that flipping is protease sensitive but does not require metabolic energy. Our assay is the first to use the endogenous substrate of the so-called 'flippase' to study phospholipid translocation in endomembranes and may therefore be crucial for the understanding of the catalytic properties of this elusive enzyme.

Details

show
hide
Language(s):
 Dates: 2000-07
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/s0014-5793(00)01684-7
PMID: 10913628
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 476 (3) Sequence Number: - Start / End Page: 277 - 281 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501