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  Cloning and overexpressing of phycoerythrocyanin operon F-gene of Mastigocladus laminosus in Escherichia coli

Zheng, M., Da, L., Deng, M., Qing, Y., Zhou, M., & Zhao, K. (2000). Cloning and overexpressing of phycoerythrocyanin operon F-gene of Mastigocladus laminosus in Escherichia coli. Acta Hydrobiologica Sinica, 26(2), 168-174.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-72EE-8 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-72F2-2
Genre: Journal Article

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Locator:
http://ssswxb.ihb.ac.cn/article/app/id/4b49730d-d505-454c-aa89-b08b314ef1aa?pageType=en (Publisher version)
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 Creators:
Zheng, M1, Author           
Da, L, Author
Deng, MG, Author
Qing, YM, Author
Zhou, M, Author
Zhao, KH, Author
Affiliations:
1External Organizations, ou_persistent22              

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 Abstract: By PCR method, apophycoeryt hrocyanin operon F-gene (p ecF) of Mastigocladus laninosus was amplified from its genom icDNA, and then cloned in pBlue-script. The pecF gene was subcloned into the expression vector pGEM D, and then transformed into E. coli BL 21 (DE3) . After induction, a new protein of molecular weight 22. 5k Da existing in inclusion body was overexpressed. The expression product was confirmed to possess phycoerythrocyanin lyase activity.

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Language(s): zho - Chinese
 Dates: 2000-03
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: -
 Degree: -

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Title: Acta Hydrobiologica Sinica
  Other : Shui sheng sheng wu xue bao
Source Genre: Journal
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Publ. Info: Beijing, China : Ke xue chu ban she
Pages: - Volume / Issue: 26 (2) Sequence Number: - Start / End Page: 168 - 174 Identifier: ISSN: 1000-3207
CoNE: https://pure.mpg.de/cone/journals/resource/1000-3207