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  Tight-Seal Whole-Cell Recording

Marty, A., & Neher, E. (1995). Tight-Seal Whole-Cell Recording. In B. Sakmann, & E. Neher (Eds.), Single-Channel Recording (pp. 31-52). New York: Springer.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-756C-8 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-756F-5
Genre: Book Chapter

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978-1-4419-1229-9_2.pdf (Publisher version), 3MB
 
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 Creators:
Marty, A.1, Author           
Neher, Erwin2, Author                 
Affiliations:
1Research Group of Cellular Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578560              
2Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: The tight-seal whole-cell recording method, often abbreviated as “whole-cell recording” (WCR), allows one to record from cells and modify their internal environment by using a patch-clamp pipette. This has become the most commonly used configuration of the patch-clamp technique. In the present chapter, we first describe the basic experimental procedures used to obtain whole-cell recordings. We then discuss the pipette—cell interactions during whole-cell recording, first from an electrical point of view and then from a chemical point of view. We finally compare the tight-seal whole-cell recording with other methods for studying electrical properties of cells.

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Language(s): eng - English
 Dates: 1995
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4419-1229-9_2
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Title: Single-Channel Recording
Source Genre: Book
 Creator(s):
Sakmann, Bert, Editor
Neher, Erwin, Editor
Affiliations:
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Publ. Info: New York : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 31 - 52 Identifier: DOI: 10.1007/978-1-4419-1229-9