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  Geometric Parameters of Pipettes and Membrane Patches

Sakmann, B., & Neher, E. (1983). Geometric Parameters of Pipettes and Membrane Patches. In B. Sakmann, & E. Neher (Eds.), Single-Channel Recording (pp. 37-51). New York: Plenum Press.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-758E-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-758F-0
Genre: Book Chapter

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978-1-4615-7858-1_2.pdf (Publisher version), 2MB
 
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 Creators:
Sakmann, Bert1, Author           
Neher, Erwin2, Author                 
Affiliations:
1Abteilung Zellphysiologie, MPI for biophysical chemistry, Max Planck Society, ou_578558              
2Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: The object of the patch current measurement is a small membrane patch and a pipette tip of comparable dimensions. Both are at the limit of resolution of the optical microscope. Only rarely is it possible to “see” the membrane patch during the experiment, and even then quantitative evaluation of its size is impossible or difficult. The purpose of this chapter is to provide some hints toward estimation of the tip geometry of patch pipettes and of patch sizes by a combination of light and electron microscopy and electrical measurements. It should be emphasized, however, that there are wide variations in tip geometry and patch sizes, even if all the parameters that are under control of the experimenter are kept constant. The numbers given should therefore only be considered as order-of-magnitude estimates.

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Language(s): eng - English
 Dates: 1983
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4615-7858-1_2
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Title: Single-Channel Recording
Source Genre: Book
 Creator(s):
Sakmann, Bert, Editor
Neher, Erwin, Editor
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Publ. Info: New York : Plenum Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 37 - 51 Identifier: ISBN: 0-306-41419-8