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  Human stem cell-derived TRPV1-positive sensory neurons: A new tool to study mechanisms of sensitization

Schrenk-Siemens, K., Pohle, J., Rostock, C., Abd El Hay, M., Lam, R. M., Szczot, M., et al. (2022). Human stem cell-derived TRPV1-positive sensory neurons: A new tool to study mechanisms of sensitization. Cells, 11(18): 2905. doi:10.3390/cells11182905.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-85C3-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-85C4-0
Genre: Journal Article

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Schrenk-Siemens_2022_HumanStemCell-Derived.pdf (Publisher version), 4MB
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Copyright Date:
2022
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Copyright © 2022 by the authors
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https://www.mdpi.com/2073-4409/11/18/2905 (Publisher version)
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 Creators:
Schrenk-Siemens, Katrin, Author
Pohle, Jörg, Author
Rostock, Charlotte, Author
Abd El Hay, Muad1, 2, Author
Lam, Ruby M., Author
Szczot, Marcin, Author
Lu, Shiying, Author
Chesler, Alexander T., Author
Siemens, Jan, Author
Affiliations:
1Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Max Planck Society, Deutschordenstr. 46, 60528 Frankfurt, DE, ou_2074314              
2Havenith & Schölvinck Lab, Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Max Planck Society, Deutschordenstraße 46, 60528 Frankfurt, DE, ou_3381231              

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Free keywords: somatosensation; human pluripotent stem cells; nociceptor-like cells; homogenous neuronal population; TRPV1 responders; translational tool
 Abstract: Somatosensation, the detection and transduction of external and internal stimuli such as temperature or mechanical force, is vital to sustaining our bodily integrity. But still, some of the mechanisms of distinct stimuli detection and transduction are not entirely understood, especially when noxious perception turns into chronic pain. Over the past decade major progress has increased our understanding in areas such as mechanotransduction or sensory neuron classification. However, it is in particular the access to human pluripotent stem cells and the possibility of generating and studying human sensory neurons that has enriched the somatosensory research field. Based on our previous work, we describe here the generation of human stem cell-derived nociceptor-like cells. We show that by varying the differentiation strategy, we can produce different nociceptive subpopulations with different responsiveness to nociceptive stimuli such as capsaicin. Functional as well as deep sequencing analysis demonstrated that one protocol in particular allowed the generation of a mechano-nociceptive sensory neuron population, homogeneously expressing TRPV1. Accordingly, we find the cells to homogenously respond to capsaicin, to become sensitized upon inflammatory stimuli, and to respond to temperature stimulation. The efficient and homogenous generation of these neurons make them an ideal translational tool to study mechanisms of sensitization, also in the context of chronic pain.

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 Dates: 2022-09-17
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/cells11182905
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Title: Cells
Source Genre: Journal
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Pages: - Volume / Issue: 11 (18) Sequence Number: 2905 Start / End Page: - Identifier: ISSN: 2073-4409