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  On the growth mechanism of the bacterial chromosome

Bonhoeffer, F., & Gierer, A. (1963). On the growth mechanism of the bacterial chromosome. Journal of Molecular Biology, 7(2), 534-540. doi:10.1016/s0022-2836(63)80100-x.

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Bonhoeffer, F, Author           
Gierer, A1, Author           
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1External Organizations, ou_persistent22              

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 Abstract: Physical methods have been used to determine the number of growth points on the DNA of E. coli bacteria. The thymine analogue [14C]5-bromouracil was incorporated into DNA for varying short times. The labelled DNA was isolated with a molecular weight of 4 × 107. The density distribution of the radioactivity was investigated in CsCl gradients.
As the size of the 5-bromouracil-labelled section of DNA increases relative to the size of the isolated piece of DNA (4 × 107 mol. wt.), the radioactivity shifts towards the density of a pure hybrid thymine—5-bromouracil double strand. From the density distribution of the radioactivity and the size of the isolated DNA one can calculate the size of the 5-bromouracil-labelled section. One calculates the same size for the total amount of radioactivity incorporated into DNA per nucleus. Thus the chromosome grows at only one point.

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 Dates: 1963-11
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: DOI: 10.1016/s0022-2836(63)80100-x
PMID: 14079592
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Title: Journal of Molecular Biology
  Other : JMB
  Abbreviation : J. Mol. Biol.
Source Genre: Journal
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Publ. Info: Elsevier
Pages: - Volume / Issue: 7 (2) Sequence Number: - Start / End Page: 534 - 540 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/0022-2836