Fast Patch-Pipette Internal Perfusion with Minimum Solution Flow

Neher, Erwin; Eckert, R.

doi:10.1007/978-1-4613-0975-8_30

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Fast Patch-Pipette Internal Perfusion with Minimum Solution Flow

Neher, E., & Eckert, R. (1988). Fast Patch-Pipette Internal Perfusion with Minimum Solution Flow. In A. D. Grinnell, D. Armstrong, & M. B. Jackson (Eds.), Calcium and Ion Channel Modulation (pp. 371-377). New York, NY: Springer.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-88AF-6 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-98C2-D

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Creators:
Neher, Erwin¹, Author
Eckert, R.¹, Author

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1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579

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Abstract: Means for changing the filling solution of patch pipettes are highly desirable whenever the influence of solution composition on cellular processes is to be studied. This refers both to patch configurations (e.g. studies of ionic selectivity or drug action on the single channel level) and to tight-seal whole cell recording. In the latter case it is particularly interesting to load cells with second messengers, regulating proteins, antibodies or toxins. Such substances very often interfere with seal formation, demanding seal formation to be performed while the pipette tip contains inert solution. A fast exchange of pipette filling solution at later times allows a timed application of the substance of interest.

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Language(s): eng - English

Dates: Date issued: 1988

Publication Status: Issued

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Rev. Type: Peer

Identifiers: DOI: 10.1007/978-1-4613-0975-8_30

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Title: Calcium and Ion Channel Modulation

Source Genre: Book

Creator(s):
Grinnell, A. D., Editor
Armstrong, D., Editor
Jackson, M. B., Editor

Affiliations:
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Publ. Info: New York, NY : Springer

Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 371 - 377 Identifier: DOI: 10.1007/978-1-4613-0975-8