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  Diet triggers specific responses of hypothalamic astrocytes in time and region dependent manner

Lutomska, L. M., Miok, V., Krahmer, N., García, I. G., Gruber, T., Le Thuc, O., et al. (2022). Diet triggers specific responses of hypothalamic astrocytes in time and region dependent manner. Glia, 70(11), 2062-2078. doi:10.1002/glia.24237.

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Glia - 2022 - Lutomska.pdf (Publisher version), 7MB
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 Creators:
Lutomska, Luiza Maria, Author
Miok, Viktorian, Author
Krahmer, Natalie, Author
García, Ismael González, Author
Gruber, Tim, Author
Le Thuc, Ophélia, Author
Murat, Cahuê DB, Author
Legutko, Beata, Author
Sterr, Michael, Author
Saher, Gesine1, Author           
Lickert, Heiko, Author
Müller, Timo D., Author
Ussar, Siegfried, Author
Tschöp, Matthias H., Author
Lutter, Dominik, Author
García-Cáceres, Cristina, Author
Affiliations:
1Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350301              

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 Abstract: How the anatomical location of these glial cells and their spatial molecular distribution in the arcuate nucleus of the hypothalamus (ARC) determine the cellular response to a high caloric diet remains unclear. In this study, we investigated their distinctive molecular responses following exposure to a high-fat high-sugar (HFHS) diet, specifically in the ARC. Using RNA sequencing and proteomics, we showed that astrocytes have a distinct transcriptomic and proteomic profile dependent on their anatomical location, with a major proteomic reprogramming in hypothalamic astrocytes. By ARC single-cell sequencing, we observed that a HFHS diet dictates time- and cell- specific transcriptomic responses, revealing that astrocytes have the most distinct regulatory pattern compared to other cell types. Lastly, we topographically and molecularly characterized astrocytes expressing glial fibrillary acidic protein and/or aldehyde dehydrogenase 1 family member L1 in the ARC, of which the abundance was significantly increased, as well as the alteration in their spatial and molecular profiles, with a HFHS diet. Together, our results provide a detailed multi-omics view on the spatial and temporal changes of astrocytes particularly in the ARC during different time points of adaptation to a high calorie diet.

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Language(s): eng - English
 Dates: 2022-07-082022-11
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/glia.24237
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Project name : The research leading to these results has received funding from European Research Council ERC (CGC: STG grant AstroNeuroCrosstalk # 757393), from the German Research Foundation DFG under Germany's Excellence Strategy within the framework of the Munich Cluster for Systems Neurology (EXC 2145 SyNergy – ID 390857198), Helmholtz Excellence Network and the Deutsche Forschungsgemeinschaft (GS: SPP1757 – SA2114/2-2), and Helmholtz Association – Initiative and Networking Fund. I.G.-G. is a recipient of a fellowship from European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie actions (842080-H2020-MSCA-IF-2018). The funders had no role in study design, data collection, analyses, decision to publish, or preparation of the manuscript. Open Access funding enabled and organized by Projekt DEAL.
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Funding organization : -
Project name : AstroNeuroCrosstalk
Grant ID : 757393
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : AstroCOMET
Grant ID : 842080
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Glia
  Other : Glia
Source Genre: Journal
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Publ. Info: New York, N.Y. : Wiley-Liss, Inc.
Pages: - Volume / Issue: 70 (11) Sequence Number: - Start / End Page: 2062 - 2078 Identifier: ISSN: 0894-1491
CoNE: https://pure.mpg.de/cone/journals/resource/954925558509