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Abstract:
Arabidposis lyrata is a close relative of model plant, A. thaliana, but the two species differ in many important life history and other traits: Outcrossing versus selfing, annual versus perennial life style, genome size and chromosome numbers. As part of the 1001 Genomes project for A. thaliana (http://1001genomes.org), we have recently sequenced 80 inbred strains of this species. Because A. lyrata is an outcrosser, resequencing natural strains is problematic, because phasing information is difficult to recover. We have therefore developed a new strategy, the sequencing of F1 hybrids between natural strains and the inbred reference strain MN47. In 32 individuals, we have identified between 0.6 and 1.3 million SNPs, which compares with an average of 0.6 million SNPs in each of the A. thaliana strains. There is a distinct genome-wide pattern of genetic differentiation between North American and European accessions. We will present inferences about how the different life histories have shaped sequence diversity in A. thaliana and A. lyrata.
