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Schlagwörter:
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Zusammenfassung:
Genome-wide sequence variation among populations reveals the history of evolutionary processes and ecological forces that molded species. In the “post-genomic” era, sequencing multiple natural strains from diverse populations has becoming increasingly desirable in a broad areas such as evolutionary, ecological and systems biology. As part of the 1001 genome project (http://1001genomes.org), which aims to sequence at least 1001 strains of Arabidopsis thaliana, we have completed a first pilot project, with 80 natural strains from across Eurasia. Each strain was sequenced to 5- to 12-fold genome coverage using Illumina’s sequencing-by-synthesis (SBS) technology. A comprehensive inventory of sequence variation including single-nucleotide polymorphisms (SNPs), small insertions/deletions (INDELs) and structural variation were generated. The effects, patterns and distribution of sequence variation have been analyzed and their population genetic implications will be discussed.
