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Abstract:
During plant development, the well-studied auxin-promoted degradation of AUX/IAA proteins that prevent ARF proteins from regulating auxin-responsive target genes is essential. However, very little is known about the transcriptional regulation of AUX/IAAs. To learn more about the transcriptional regulation of the IAA12/BDL gene, which is expressed in the apical daughter cell of the zygote and its progeny, we analyzed the BDL promoter. An initial in silico analysis followed by in planta validation revealed an evolutionarily conserved promoter fragment that is necessary and sufficient for BDL expression. Here, we present results on regulator Of BDL1 (ROB1), which functions as a direct repressor of BDL expression during embryo and root development. During embryo development, the spatial regulation of BDL expression appears to be crucial for the formation of cotyledons. Taken together, our results suggest that not only auxin-dependent degradation of AUX/IAAs controls auxin responses, but that also auxin-independent transcriptional regulation of AUX/IAAs is crucial for development.
