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  Solutions of Supramolecular Assemblies in the Magic-Angle-Spinning Rotor

Mainz, A., Religa, T., Sprangers, R., Linser, R., Kay, L., & Reif, B. (2013). Solutions of Supramolecular Assemblies in the Magic-Angle-Spinning Rotor. Poster presented at 35th FGMR Discussion Meeting & Joint Conference of the German, Italian and Slovenian Magnetic Resonance Societies, Frauenchiemsee, Germany.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-C282-5 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-C284-3
Genre: Poster

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https://iris.unito.it/retrieve/e27ce428-ed68-2581-e053-d805fe0acbaa/joint_conference_2013.pdf (Abstract)
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Mainz, A, Author
Religa, TL, Author
Sprangers, R1, Author           
Linser, R, Author
Kay, LE, Author
Reif, B, Author
Affiliations:
1Research Group NMR Spectroscopy of Large Complexes, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3376932              

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 Abstract: Various processes in the living cell are regulated by very large protein machineries with molecular weights in the megadalton regime. However, structural investigations of these large systems by solution-state NMR are challenging due to their slow rotational diffusion. Here we describe an approach, which opens new perspectives in the investigation of soluble supramolecular modules by magic-angle spinning (MAS) NMR. The absence of rotational diffusion in MAS-induced protein sediments allows typical solid-state NMR techniques without the need of invasive precipitation or crystallization procedures. The combination with protein deuteration, proton-detection and paramagnetic relaxation enhancement enabled us to observe and to assign backbone amide resonances of a 20S proteasome assembly with a molecular weight of 1.1 MDa. Similarly, we used the approach to characterize the polydisperse and highly dynamic small heat-shock protein αB-crystallin (600 kDa) with respect to its copper-dependent chaperone activity and its mechanism of binding destabilized substrate proteins.

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 Dates: 2013-09
 Publication Status: Published online
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Title: 35th FGMR Discussion Meeting & Joint Conference of the German, Italian and Slovenian Magnetic Resonance Societies
Place of Event: Frauenchiemsee, Germany
Start-/End Date: 2013-09-09 - 2013-09-14

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Title: 35th FGMR Discussion Meeting & Joint Conference of the German, Italian and Slovenian Magnetic Resonance Societies
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: P4 Start / End Page: 113 Identifier: -