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  Crystal structure of the colicin M immunity protein

Braun, V., Helbig, S., Patzer, S., Römer, C., & Zeth, K. (2011). Crystal structure of the colicin M immunity protein. Poster presented at Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM 2012), Karlsruhe, Germany.

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Braun, V1, Author                 
Helbig, S1, Author           
Patzer, S1, Author                 
Römer, C1, Author           
Zeth, K1, Author                 
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1Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375791              

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 Abstract: Colicins are bacterial protein toxins produced by half of E. coli natural isolates that kill sensitive E. coli cells. Colicin M (Cma) inhibits incorporation of murein precursors into murein. Cma producer cells are protected by co-synthesis of an immunity protein, Cmi, that is located at the Cma target site in the periplasm and anchored to the cytoplasmic membrane by an N-terminal hydrophobic sequence [1]. We resumed our previous studies on Cma and Cmi after we had discovered that Cma activity requires the periplasmic FkpA prolyl cis-trans isomerase /chaperone [2]. Since the hydrophobic sequence is not essential for Cmi activity [1], crystallization was performed with a soluble Cmi that lacked the N- terminus. Cmi crystals were obtained under several conditions but only one single crystal diffracted to a resolution of 1.95 Å. By using the recently published software package ARCIMBOLDO [3], we succeeded to solve the structure by this de novo approach (Dayté Rodriguez, Isabel Usón- Finkenzeller, Instituto di Biología Molecular de Barcelona, Barcelona, Spain). In the crystal Cmi forms a dimer that is interlinked by a disulfide bridge. It is a highly charged protein with a surplus of negative charges presumably responsible for interaction with Cma which contains a cluster of positive charges.

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 Dates: 2011-04
 Publication Status: Issued
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Title: Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM 2012)
Place of Event: Karlsruhe, Germany
Start-/End Date: 2011-04-03 - 2011-04-06

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Title: Biospektrum
Source Genre: Journal
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Publ. Info: Heidelberg, Germany : Spektrum Akademischer Verlag
Pages: - Volume / Issue: 2011 (Sonderausgabe) Sequence Number: CBP039 Start / End Page: 81 - 82 Identifier: ISSN: 0947-0867
CoNE: https://pure.mpg.de/cone/journals/resource/110978984077563