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  Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR

Pantoja, C., Ibáñez de Opakua, A., Cima-Omori, M.-S., & Zweckstetter, M. (2023). Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR. Angewandte Chemie International Edition, 62(17): e202218078. doi:10.1002/anie.202218078.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-F5AA-0 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-F5AB-F
Genre: Journal Article

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Pantoja, C.F., Author
Ibáñez de Opakua, A., Author
Cima-Omori, M.-S., Author
Zweckstetter, Markus1, 2, Author           
Affiliations:
1Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350124              
2Research Group of Protein Structure Determination using NMR, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350128              

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 Abstract: Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of spatially-resolved NMR to condensates formed by the Alzheimer's disease-associated protein Tau demonstrates decreased water content, exclusion of the molecular crowding agent dextran, presence of a specific chemical environment of the small molecule DSS, and ≈150-fold increased concentration of Tau inside the condensate. The results suggest that spatially-resolved NMR can have a major impact in understanding the composition and physical chemistry of biomolecular condensates.

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Language(s): eng - English
 Dates: 2023-02-272023-04-17
 Publication Status: Issued
 Pages: -
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 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202218078
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Project name : C.F.P. acknowledges the Deutscher Akademischer Austauschdienst (DAAD) for the Research Grants Doctoral program in Germany, 2019/20 (personal ref. no. 91726791). M.Z. was supported by the European Research Council (ERC) under the EU Horizon 2020 research and innovation program (grant agreement no. 787679).
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Project name : LLPS-NMR
Grant ID : 787679
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 62 (17) Sequence Number: e202218078 Start / End Page: - Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851