English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Comprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components

Ugur, E., de la Porte, A., Qin, W., Bultmann, S., Ivanova, A., Drukker, M., et al. (2023). Comprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components. Nucleic Acids Research. doi:10.1093/nar/gkad058.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Ugur, Enes1, Author           
de la Porte, Alexandra2, Author
Qin, Weihua2, Author
Bultmann, Sebastian2, Author
Ivanova, Alina2, Author
Drukker, Micha2, Author
Mann, Matthias1, Author           
Wierer, Michael1, Author           
Leonhardt, Heinrich2, Author
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

Content

show
hide
Free keywords: DATA-INDEPENDENT ACQUISITION; STEM-CELL TRANSITION; NAIVE PLURIPOTENCY; PROFILING REVEALS; MOUSE EPIBLAST; PROTEINS; STATE; HBO1; PROGRESSION; ENRICHMENTBiochemistry & Molecular Biology;
 Abstract: The establishment of cellular identity is driven by transcriptional and epigenetic regulators of the chromatin proteome - the chromatome. Comprehensive analyses of the chromatome composition and dynamics can therefore greatly improve our understanding of gene regulatory mechanisms. Here, we developed an accurate mass spectrometry (MS)-based proteomic method called Chromatin Aggregation Capture (ChAC) followed by Data-Independent Acquisition (DIA) and analyzed chromatome reorganizations during major phases of pluripotency. This enabled us to generate a comprehensive atlas of proteomes, chromatomes, and chromatin affinities for the ground, formative and primed pluripotency states, and to pinpoint the specific binding and rearrangement of regulatory components. These comprehensive datasets combined with extensive analyses identified phase-specific factors like QSER1 and JADE1/2/3 and provide a detailed foundation for an in-depth understanding of mechanisms that govern the phased progression of pluripotency. The technical advances reported here can be readily applied to other models in development and disease.

Details

show
hide
Language(s): eng - English
 Dates: 2023-02-20
 Publication Status: Published online
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000935321900001
DOI: 10.1093/nar/gkad058
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic Acids Research
  Other : Nucleic Acids Res.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0301-5610
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000262810