English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Adhesion GPCR Latrophilin 3 regulates synaptic function of cone photoreceptors in a trans-synaptic manner

Wang, Y., Cao, Y., Hays, C. L., Laboute, T., Ray, T. A., Guerrero-Given, D., et al. (2021). Adhesion GPCR Latrophilin 3 regulates synaptic function of cone photoreceptors in a trans-synaptic manner. Proceedings of the National Academy of Sciences, (45). Retrieved from https://www.pnas.org/content/118/45/e2106694118.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-000C-DFBE-4 Version Permalink: https://hdl.handle.net/21.11116/0000-000C-DFBF-3
Genre: Journal Article
Alternative Title : Proceedings of the National Academy of Sciences

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Wang, Yuchen, Author
Cao, Yan, Author
Hays, Cassandra L., Author
Laboute, Thibaut, Author
Ray, Thomas A., Author
Guerrero-Given, Debbie1, Author
Ahuja, Abhimanyu S., Author
Patil, Dipak, Author
Rivero, Olga, Author
Kamasawa, Naomi1, Author
Kay, Jeremy N., Author
Thoreson, Wallace B., Author
Martemyanov, Kirill A., Author
Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288              

Content

show
hide
Free keywords: adhesion GPCR, horizontal cells, retina, synapses, vision
 Abstract: Cone photoreceptors mediate daylight vision in vertebrates. Changes in neurotransmitter release at cone synapses encode visual information and is subject to precise control by negative feedback from enigmatic horizontal cells. However, the mechanisms that orchestrate this modulation are poorly understood due to a virtually unknown landscape of molecular players. Here, we report a molecular player operating selectively at cone synapses that modulates effects of horizontal cells on synaptic release. Using an unbiased proteomic screen, we identified an adhesion GPCR Latrophilin3 (LPHN3) in horizontal cell dendrites that engages in transsynaptic control of cones. We detected and characterized a prominent splice isoform of LPHN3 that excludes a element with inhibitory influence on transsynaptic interactions. A gain-of-function mouse model specifically routing LPHN3 splicing to this isoform but not knockout of LPHN3 diminished CaV1.4 calcium channel activity profoundly disrupted synaptic release by cones and resulted in synaptic transmission deficits. These findings offer molecular insight into horizontal cell modulation on cone synaptic function and more broadly demonstrate the importance of alternative splicing in adhesion GPCRs for their physiological function.

Details

show
hide
Language(s):
 Dates: 2021
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: URI: https://www.pnas.org/content/118/45/e2106694118
URI: https://www.pnas.org/doi/pdf/10.1073/pnas.2106694118
URI: http://www.ncbi.nlm.nih.gov/pubmed/34732574
URI: https://www.pnas.org/content/118/45/e2106694118
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences
  Alternative Title : PNAS
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: (45) Sequence Number: - Start / End Page: - Identifier: ISBN: 0027-8424, 1091-6490