A deep learning approach to identifying immunogold particles in electron 
microscopy images

Jerez, Diego; Stuart, Eleanor; Schmitt, Kylie; Guerrero-Given, Debbie; Christie, Jason M.; Kamasawa, Naomi; Smirnov, Michael S.

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A deep learning approach to identifying immunogold particles in electron microscopy images

Jerez, D., Stuart, E., Schmitt, K., Guerrero-Given, D., Christie, J. M., Kamasawa, N., et al. (2021). A deep learning approach to identifying immunogold particles in electron microscopy images. Scientific Reports, (1). Retrieved from https://www.nature.com/articles/s41598-021-87015-2.

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Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-000C-DFC8-8 Versions-Permalink: https://hdl.handle.net/21.11116/0000-000C-DFC9-7

Genre: Zeitschriftenartikel

Alternativer Titel : Scientific Reports

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Urheber:
Jerez, Diego¹, Autor
Stuart, Eleanor¹, Autor
Schmitt, Kylie¹, Autor
Guerrero-Given, Debbie¹, Autor
Christie, Jason M.¹, Autor
Kamasawa, Naomi¹, Autor
Smirnov, Michael S.¹, Autor

Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288

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Zusammenfassung: Electron microscopy (EM) enables high-resolution visualization of protein distributions in biological tissues. For detection, gold nanoparticles are typically used as an electron-dense marker for immunohistochemically labeled proteins. Manual annotation of gold particle labels is laborious and time consuming, as gold particle counts can exceed 100,000 across hundreds of image segments to obtain conclusive data sets. To automate this process, we developed Gold Digger, a software tool that uses a modified pix2pix deep learning network capable of detecting and annotating colloidal gold particles in biological EM images obtained from both freeze-fracture replicas and plastic sections prepared with the post-embedding method. Gold Digger performs at near-human-level accuracy, can handle large images, and includes a user-friendly tool with a graphical interface for proof reading outputs by users. Manual error correction also helps for continued re-training of the network to improve annotation accuracy over time. Gold Digger thus enables rapid high-throughput analysis of immunogold-labeled EM data and is freely available to the research community.