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Schlagwörter:
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Zusammenfassung:
Fluorescent microscopy is the primary method to study DNA organization
within cells. However the variability and low signal-to-noise commonly
associated with live-cell time lapse imaging challenges quantitative
measurements. In particular, obtaining quantitative or mechanistic
insight often depends on the accurate tracking of fluorescent particles.
Here, we present ★Track, an inference method that determines the most
likely temporal tracking of replicating intracellular particles such DNA
loci while accounting for missing, merged and spurious detections. It
allows the accurate prediction of particle copy numbers as well as the
timing of replication events. We demonstrate ★Track's abilities and gain
new insight into plasmid copy number control and the volume dependence
of bacterial chromosome replication initiation. By enabling the accurate
tracking of DNA loci, ★Track can help to uncover the mechanistic
principles of chromosome organisation and dynamics across a range of
systems.