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  A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins

Umebayashi, M., Takemoto, S., Reymond, L., Sundukova, M., Hovius, R., Bucci, A., et al. (2023). A covalently linked probe to monitor local membrane properties surrounding plasma membrane proteins. The Journal of Cell Biology: JCB, 222(3): e202206119, pp. 1-22. doi: 10.1083/jcb.202206119.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000C-EA72-C Version Permalink: https://hdl.handle.net/21.11116/0000-000D-0B47-8
Genre: Journal Article

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 Creators:
Umebayashi, Miwa, Author
Takemoto, Satoko, Author
Reymond, Luc, Author
Sundukova, Mayya, Author
Hovius, Ruud, Author
Bucci, Annalisa, Author
Heppenstall, Paul A, Author
Yokota, Hideo, Author
Johnsson, Kai1, Author           
Riezman, Howard, Author
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: Functional membrane proteins in the plasma membrane are suggested to have specific membrane environments that play important roles to maintain and regulate their function. However, the local membrane environments of membrane proteins remain largely unexplored due to the lack of available techniques. We have developed a method to probe the local membrane environment surrounding membrane proteins in the plasma membrane by covalently tethering a solvatochromic, environment-sensitive dye, Nile Red, to a GPI-anchored protein and the insulin receptor through a flexible linker. The fluidity of the membrane environment of the GPI-anchored protein depended upon the saturation of the acyl chains of the lipid anchor. The local environment of the insulin receptor was distinct from the average plasma membrane fluidity and was quite dynamic and heterogeneous. Upon addition of insulin, the local membrane environment surrounding the receptor specifically increased in fluidity in an insulin receptor-kinase dependent manner and on the distance between the dye and the receptor.

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Language(s): eng - English
 Dates: 2022-09-212022-06-282022-12-092022-12-262023-03-06
 Publication Status: Issued
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: URI: https://pubmed.ncbi.nlm.nih.gov/36571579/
DOI: 10.1083/jcb.202206119
 Degree: -

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Title: The Journal of Cell Biology : JCB
  Other : J. Cell Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 222 (3) Sequence Number: e202206119 Start / End Page: 1 - 22 Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024