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  Sequence-specific DNA labelling for fluorescence microscopy

Pradhan, S., Apaydin, S., Bucevičius, J., Gerasimaitė, R., Kostiuk, G., & Lukinavičius, G. (2023). Sequence-specific DNA labelling for fluorescence microscopy. Biosensors and Bioelectronics, 230: 115256. doi:10.1016/j.bios.2023.115256.

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1-s2.0-S0956566323001987-main.pdf (Publisher version), 10MB
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Version of Record: 27 March 2023
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 Creators:
Pradhan, Shalini1, 2, Author           
Apaydin, Sinem1, 2, Author           
Bucevičius, Jonas1, 2, Author           
Gerasimaitė, Rūta1, 2, Author           
Kostiuk, Georgij1, 2, Author           
Lukinavičius, Gražvydas1, 2, Author           
Affiliations:
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350048              
2Research Group of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350123              

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 Abstract: The preservation of nucleus structure during microscopy imaging is a top priority for understanding chromatin organization, genome dynamics, and gene expression regulation. In this review, we summarize the sequence-specific DNA labelling methods that can be used for imaging in fixed and/or living cells without harsh treatment and DNA denaturation: (i) hairpin polyamides, (ii) triplex-forming oligonucleotides, (iii) dCas9 proteins, (iv) transcription activator-like effectors (TALEs) and (v) DNA methyltransferases (MTases). All these techniques are capable of identifying repetitive DNA loci and robust probes are available for telomeres and centromeres, but visualizing single-copy sequences is still challenging. In our futuristic vision, we see gradual replacement of the historically important fluorescence in situ hybridization (FISH) by less invasive and non-destructive methods compatible with live cell imaging. Combined with super-resolution fluorescence microscopy, these methods will open the possibility to look into unperturbed structure and dynamics of chromatin in living cells, tissues and whole organisms.

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Language(s): eng - English
 Dates: 2023-03-212023-06-15
 Publication Status: Issued
 Pages: -
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bios.2023.115256
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Title: Biosensors and Bioelectronics
  Other : Biosens. Bioelectron.
Source Genre: Journal
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Publ. Info: Amsterdam u. a. : Elsevier
Pages: - Volume / Issue: 230 Sequence Number: 115256 Start / End Page: - Identifier: ISSN: 0956-5663
CoNE: https://pure.mpg.de/cone/journals/resource/954925577034