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  Optimization and characterization of the synthetic secondary chromosome synVicII in Escherichia coli

Messerschmidt, S. J., Schindler, D., Zumkeller, C. M., Kemter, F. S., Schallopp, N., & Waldminghaus, T. (2016). Optimization and characterization of the synthetic secondary chromosome synVicII in Escherichia coli. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 4: 96. doi:10.3389/fbioe.2016.00096.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000D-077F-E Version Permalink: https://hdl.handle.net/21.11116/0000-000D-0780-A
Genre: Journal Article

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 Creators:
Messerschmidt, Sonja J.1, Author
Schindler, Daniel2, Author                 
Zumkeller, Celine M.1, Author
Kemter, Franziska S.1, Author
Schallopp, Nadine1, Author
Waldminghaus, Torsten1, Author
Affiliations:
1external, ou_persistent22              
2LOEWE-Center for Synthetic Microbiology (SYNMIKRO), Philipps-Universität Marburg, Germany, ou_persistent22              

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 Abstract: Learning by building is one of the core ideas of synthetic biology
research. Consequently, building synthetic chromosomes is the way to
fully understand chromosome characteristics. The last years have seen
exciting synthetic chromosome studies. We had previously introduced the
synthetic secondary chromosome synVicII in Escherichia coli. It is based
on the replication mechanism of the secondary chromosome in Vibrio
cholerae. Here, we present a detailed analysis of its genetic
characteristics and a selection approach to optimize replicon stability.
We probe the origin diversity of secondary chromosomes from Vibrionaceae
by construction of several new respective replicons. Finally, we present
a synVicII version 2.0 with several innovations including its full
compatibility with the popular modular cloning (MoClo) assembly system.

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 Dates: 2016-12-23
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000390523300001
DOI: 10.3389/fbioe.2016.00096
 Degree: -

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Title: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 4 Sequence Number: 96 Start / End Page: - Identifier: ISSN: 2296-4185